Abstract

The aim of study was to investigate the virulence factors of phospholipase, proteinase, esterase production and biofilm formation in Candida species isolated from patients with candidemia, and to assess their relationship with Candida genotypes derived after repetitive sequence-based polymerase chain reaction (rep-PCR) fingerprinting. Fifty-two strains were identified to species level according to conventional methods and sequencing. The DiversiLab system was used for the genotyping. Enzyme activities and biofilm formation were evaluated using microbiological methods. The 52 strains were identified as follows: 29 C. parapsilosis, 19 C. albicans, 2 C. glabrata, and 2 C. tropicalis. Phospholipase and proteinase activities were observed to have statistically significant differences between C. albicans and non-albicans Candida (NAC) strains (p < 0.05), with C. albicans strains showing higher virulence. Rep-PCR revealed eight major genotypes (A-H).The 19 C. albicans and the 33 non-albicans Candida isolates yielded seven (A-G) and four (A, B, C, H) genotypes, respectively. C. albicans strains were not shown to have a predominant genotype and showed higher phospholipase and proteinase activitiy than did NAC, regardless of genotype. Genotype H (52%) was the predominant genotype for the NAC including 27 C. parapsilosis strains, but the majority of strains showed low virulence. NAC species were the most common causative agent for candidemia. Genotyping showed low transmission of C. albicans strains, but transmission of C. parapsilosis was high. In candidemia, several Candida virulence factors may be responsible at the same time. However, different genotypes of Candida strains showed different virulence activity.

Highlights

  • The aim of study was to investigate the virulence factors of phospholipase, proteinase, esterase production and biofilm formation in Candida species isolated from patients with candidemia, and to assess their relationship with Candida genotypes derived after repetitive sequence-based polymerase chain reaction fingerprinting

  • C. albicans is most often associated with invasive fungal infection, non-albicans Candida species have emerged as clinically important pathogens [5,6,7,8,9,10,11]

  • When groups were divided according to C. albicans and non-albicans Candida, the phospholipase, proteinase, esterase activities and biofilm formation were observed in 18 (94.7%), 14 (73.7%), 5 (26.3%), and 5 (26.3%) C. albicans and in 2 (6%), 11 (33.3%), 9 (27.2%), and 10 (30.3%) non-albicans Candida isolates, respectively (Table 2)

Read more

Summary

Introduction

The aim of study was to investigate the virulence factors of phospholipase, proteinase, esterase production and biofilm formation in Candida species isolated from patients with candidemia, and to assess their relationship with Candida genotypes derived after repetitive sequence-based polymerase chain reaction (rep-PCR) fingerprinting. The main virulence factors of Candida spp. that have been studied are production of phospholipase, proteinase, esterase and biofilm formation. There are many methods used for epidemiological typing, such as multi-locus enzyme electrophoresis, electrophoretic karyotyping, random amplified polymorphic DNA, microsatellite length polmorphism, pulsed-field gel electrophoresis (PFGE), and multi-locus sequence typing. These methods are not practical for the clinical laboratory because they are time consuming and labor intensive. The discriminatory power of rep-PCR is good [12]

Objectives
Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call