Investigation of the Protective Effect of Terebinth (Pistacia terebinthus L.) against Ovarian Ischemia-Reperfusion Damage in Rat

Abstract

The aim of this study is to investigate the therapeutic and protective effects of Pistacia terebinthus L. (terebinth) oil treatment on experimental ovarian ischemia and reperfusion injury in rats. In the study, 32 Sprague-Dawley female rats; They were divided into 4 groups: control, ischemia, ischemia/reperfusion (I/R), ischemia/reperfusion + terebinth oil (I/R + terebinth oil), 8 in each group. Terebinth oil was applied via oral gavage at a daily dose of 2 ml/kg for 4 weeks. In histopathological examination of ovarian sections, the control group showed a regular histological appearance. In the ischemia and I/R groups, degenerations in the epithelium, inflammatory cell infiltration in the lamina propria region, and bleeding due to thrombosis in the enlarged vessels were observed. It was determined that terebinth oil protected the epithelial tissue after ovarian I/R injury, reduced inflammation in the connective tissue, and brought the artery-vein contours to a regular appearance. In the ovarian sections where immunohistochemistry analysis was performed, it was observed that radixin protein expression showed a positive reaction in the granulosa cells and a negative reaction in the stromal areas in the terebinth oil applied group compared to the I/R group. As a result, it was revealed that terebinth oil has a healing and protective effect in the treatment of ovarian ischemia/reperfusion injury.
 Keywords: Terebinth (Pistacia terebinthus L), Ovary, Ischemia, Reperfusion