Investigation of the Prevalence of Brucella Diagnosis in Serum and Milk in Cattle

Abstract

Background: Brucellosis is an infectious disease that affects both cattle and humans. Because brucellosis causes economic losses, serovalance studies are important in terms of eradication. Its diagnosis is integral to the design and implementation of preventive and management strategies for both cattle and humans. Various serological tests are commonly used in diagnostic processes. Methods:This study was carried out in cattle farms located in Ankara, Çankırı, Kırşehir, Kayseri, Çorum, Yozgat, Kırıkkale, Nevşehir provinces in the Central Anatolia region of Turkey between January-2018 and December-2020. Bovines included in the study, simple random sampling method and 320 bovine blood serum and milk samples that were reported not to have Brucella vaccine were used. RBPT, SAT, CFT, I-ELISA tests were performed in the blood serum samples included in the study and I-Elisa, MRT tests were performed in the milk samples. Result: The tests revealed that an average of 35.4% of the cattle had the bacteria while 64.5% of the raw milk samples were infected with brucellosis. The findings demonstrated the need to implement better strategies for managing and preventing brucellosis among animals, including vaccination. As a result, indirect ELISA (I-ELISA) method is considered as a useful, reliable, fast tool in the detection of Brucella from in milk and serum samples. However, it I-ELISA method can be used as a support for conventional tests in the initial isolation of waste materials and that its simultaneous use in field screening tests can be good diagnostic performance.