Abstract
Purpose : To investigate the potential role of checkpoint kinase2 (CHEK2) in regulating gastric cancer stem cells. Methods : The abnormal features of cellular arrangements in early and advanced stages of gastric cancer were analysed using histology. Immunohistochemistry and Western blotting were used to evaluate the expressions of cancer stem cell markers CD44 and CHEK2 at different stages of gastric cancer. Results : When compared with the control tissue, the size of the cellular nucleus was enlarged as the gastric tumour developed to more advanced stages, and the expression of CD44 increased exponentially. The expression of CHEK2, a protein that regulates cell cycle and apoptosis was aberrantly up-regulated at the early stage of cancer, but as the tumour advanced, its expression became down-regulated. Conclusion : These results show that the higher expression of CHEK2 at the early stages of gastric cancer exerts control over gastric cancer stem cell proliferation. Thus, CHECK2 is a potential target for early treatment of gastric cancer. Keywords : Gastric cancer, CD44, CHEK2, Gastric cancer stem cells
Highlights
Gastric cancer is a tumour that develops inside the lining of the stomach, and it constitutes a major health problem worldwide [1,2]
The present study was carried out to investigate the level of expression of checkpoint kinase2 (CHEK2) and markers of gastric cancer stem cell so as to identify their association with cancer progression
The expression of CD44 showed an up-regulated pattern as the tumour progressed to the advanced stage, but CHEK2 expression was down-regulated (Figure 3, Lane 3)
Summary
Gastric cancer is a tumour that develops inside the lining of the stomach, and it constitutes a major health problem worldwide [1,2]. When it is not treated at the early stage, it can spread to other organs in the body like liver, abdomen, lungs, bones and lymph nodes [3]. The present study was carried out to investigate the level of expression of CHEK2 and markers of gastric cancer stem cell so as to identify their association with cancer progression. The patients were examined, and gastric cancer tissue samples were removed for further analysis. Values of p less than 0.05 were considered as significant
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