Investigation of the Possible Cytopathological Effect of Human Papillomavirus Infection on p-16INK4a Overexpressed Urothelial Carcinomas of the Bladder in the Urine

Seiko Ogura Toshitetsu

doi:10.4172/2157-7099.1000335

Abstract

Background and objectives: Human papillomavirus (HPV) is well-known as the etiological agent for uterine cervical cancer or carcinoma of the oropharynx, anus and vulva. However, the cytopathologic effect of the HPV infection in urinary bladder tumors has rarely been reported. The present study has been carried out to search for cytopathologic differences between HPV positive and negative cases with urothelial carcinoma. Methods: We examined 91 specimens from 63 patients who underwent transurethral resection or biopsy for papillary urothelial carcinomas between May 2010 and September 2012. p-16INK4a expression was evaluated by immunohistochemistry. Detection of HPV DNA was carried out by in situ hybridization on formalin-fixed, paraffinembedded tissue sections. Urine smears were compared cytomorphologically between HPV-positive and HPVnegative cases. Results: p-16INK4a overexpression was detected in 29 cases (31.9%, 29/91). Of them, HPV DNA was detected in 11 cases. No significant cytopathologic differences were found in tumor cells when HPV-positive and HPV-negative cases were compared. Conclusion: HPV could be detected in urothelial carcinomas of the urinary bladder. There were no significant differences in cytopathologic features of urine smears between HPV-positive and HPV-negative cases with urothelial carcinomas. These results suggest that HPV infection in urinary tract does not add substantial clinically relevant importance to the carcinogenesis and cytomorphology of urothelial carcinomas.

Highlights

Human papillomavirus (HPV) is well-known as the major etiological agent for uterine cervical cancer
The most of their studies have focused on the detection of HPV infection, and little attention has been paid to the cytologic morphology correlated with HPV infection in urinary bladder tumor
Detection of HPV-DNA and overexpression of p16 INK4a has been reported at a high rate in cervical squamous cell carcinoma, but there are only occasional reports of HPV-DNA and p16 INK4a expression in urothelial carcinoma of the bladder

Summary

Introduction

Human papillomavirus (HPV) is well-known as the major etiological agent for uterine cervical cancer. Detection of HPV-DNA and overexpression of p16 INK4a has been reported at a high rate in cervical squamous cell carcinoma, but there are only occasional reports of HPV-DNA and p16 INK4a expression in urothelial carcinoma of the bladder. The present study has been carried out to search for cytopathologic differences between HPV positive and negative cases with urothelial carcinoma and the possible role of p16 expression in the carcinogenesis of urothelial carcinoma or association with the HPV infection. Human papillomavirus (HPV) is well-known as the etiological agent for uterine cervical cancer or carcinoma of the oropharynx, anus and vulva. The present study has been carried out to search for cytopathologic differences between HPV positive and negative cases with urothelial carcinoma

Methods

Results

Conclusion