Investigation of the pathogenesis of cell fusion in multiple myeloma bone disease

Abstract

Objective To discuss function of the fusion cells of human bone marrow stromal cells (BMSCs) and human myeloma cell RPMI 8226 in the pathogenesis of multiple myeloma bone disease. Methods The cells , labeled by cell tracer green fluorescent probe (CMFDA) and red fluorescent probe (CMTMR), respectively, were induced into fusion by chemical polyethylene glycol (polyethyleneglycol, PEG-1000), and cell fusion model was set up. Whether fusion cells had nucleus fusion was determined by Karyotype analysis. The expressions of stemness-related genes , SIRPα gene and DC-STAMP gene in fusion cells were identified. Results Polyethylene glycol (PEG-1000) could mediate the integration of BMSCs and RPMI 8226 cells.The number of chromosomes in more than 80 % the hybrid cells was about 80. Fusion cells not only showed that BMSCs, stemness-related genes of c-myc, Klf-4 and OCT-4 genes expressed positively, but also the fusion-related genes SIRPα and DC-STAMP expressed positively. Conclusion BMSCs and RPMI 8226 cells can form fusion cells, and the cells have the potential for further integration, which is one of the important reasons for the promotion of multiple myeloma bone destruction. Key words: Cell fusion; Multiple myeloma; Myeloma bone disease; Polyethyleneglycol