Investigation of the neighbour relationships between photosystem II polypeptides in the two types of isolated reaction centres (D1/D2/cyt b559 and CP47/D1/D2/cyt b559 complexes)

Andrey A Moskalenko,Roberto Barbato,Giorgio M Giacometti

doi:10.1016/0014-5793(92)81487-7

Investigation of the neighbour relationships between photosystem II polypeptides in the two types of isolated reaction centres (D1/D2/cyt b559 and CP47/D1/D2/cyt b559 complexes)

Andrey A Moskalenko, Roberto Barbato + Show 1 more

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https://doi.org/10.1016/0014-5793(92)81487-7

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Journal: FEBS Letters	Publication Date: Dec 21, 1992
Citations: 29

Affiliation: University of Padua

#Illumination Of Complexes #Light Of High Intensity + Show 8 more

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Abstract

The nearest neighbour relationships within the D1/D2/cyt b 559 complex (PSIIRC) and the CP47/D1/D2/cyt b 559 complex (RC-CP47) were investgated by using different length bifunctional crosslinking agents. The crosslinking products were identified by immunoblotting with polyclonal antibodies and by two-dimensional gel electrophoresis. Seven products (CP47/D2, D1/D2/α, D1/D2, D2/α, D1/α, α/αa, α/β) have been revealed in both complexes. The crosslinking of both complexes does not increase their photostability. The photocrosslinking products (D1/α and D2/α) appeared under illumination of complexes with light of high intensity.

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