Investigation of the Microbiological Relationship between Periodontal Pocket and Root Canal of the Teeth with Furcation Defect and Endodontic Problem using PCR Analysis

Abstract

It was shown that the factors cause periodontal diseases also lead endodontic problems. The aim of this study is to investigate the primer periodontally seconder endodontic infected non-carious teeth with furcation defects, microbiologically, using polymerase chain reaction (PCR). The study included 24 molar teeth of 24 patients with primer periodontally seconder endodontic problems. Plaque and gingival indexes, pocket depths, furcation defect and mobility of each tooth were recorded. After isolation of the tooth, subgingival plaque samples and microbiological samples from the root canal were collected with the use of sterile paper points. DNA isolation and PCR analysis were performed according to manufacturer’s instructions and special primers of T. forsythia, F. nucleatum, P. gingivalis, P. endodontalis, T. denticola, E. feacalisand A. actinomycetemcomitans were used. The deepest and smallest pockets were 10 and 6mm, respectively. 75% of the teeth had Class 2 and 25% of teeth had Class 3 furcation defects. P.gingivalis, P.endodontalisand T. Denticola were detected in both pocket and root canal, significantly. There was a significant correlation between increasing the pocket dept and presence of the T. forsythia, F. nucleatum, P.gingivalis, P. endodontalisand T. denticolain subgingival plaque. The success of periodontal and endodontic treatments is closely related to elimination of microorganisms. Within the limitations of this study, periodontal disease of teeth with Class 2 and Class 3 furcation defects leads to secondary endodontic infection. Endodontic treatment of these teeth can be achieved by the elimination of periodontal problems.