Abstract
Objective: to analyze the 3D micro- and nano-structure and quantitative morphological parameters of liver cells cultured on biodegradable silk fibroin-based film scaffolds. Materials and methods . Samples of biodegradable silk fibroin-based scaffolds with cultured Wistar rat liver cells were obtained for the study. The 3D structure of liver cells cultivated on the scaffolds was studied by scanning probe optical nanotomography using an experimental setup combining an ultramicrotome and a scanning probe microscope in correlation with fluorescence microscopy. Results . Nanoscale images and 3D nanotomographic reconstructions of rat liver cells cultured on scaffold were obtained. The morphological parameters of liver cells (average roughness, specific effective area) were determined. The average surface roughness of the liver cells R a was found to be 124.8 ± 8.2 nm, while the effective surface area σ was 1.13 ± 0.02. Analysis of the volume distribution of lipid droplets showed that they occupy 28% of the cell volume . Conclusion. Scanning probe optical nanotomography can successfully analyze the nanostructure and quantify the nanomorphology of liver cells cultured on biodegradable scaffolds.
Highlights
Investigation of the micro- and nano-structure of liver cells cultured on biodegradable silk fibroin‐based scaffolds using scanning probe optical nanotomography
Objective: to analyze the 3D micro- and nano-structure and quantitative morphological parameters of liver cells cultured on biodegradable silk fibroin-based film scaffolds
Samples of biodegradable silk fibroin-based scaffolds with cultured Wistar rat liver cells were obtained for the study
Summary
Исследование микро- и наноструктуры клеток печени, культивированных на биодеградируемых скаффолдах на основе фиброина шелка, методом сканирующей зондовой оптической нанотомографии. Провести анализ трехмерной микро- и наноструктуры и количественных морфологических параметров клеток печени, культивированных на биодеградируемых скаффолдах в виде пленок на основе фиброина шелка. Были получены образцы биодеградируемых скаффолдов на основе фиброина шелка с культивированными клетками печени крысы породы Wistar. Полученные в результате исследования данные демонстрируют, что технология сканирующей зондовой оптической нанотомографии позволяет успешно анализировать наноструктуру и определять количественные характеристики наноморфологии клеток печени, культивированных на биодеградируемых скаффолдах. В качестве объектов настоящего исследования были выбраны биодеградируемые скаффолды на основе фиброина шелка в виде пленок с культивированными клетками печени крысы. В рамках данной работы выполнены исследования микро- и наноструктуры клеток печени крысы, адгезированных на биодеградируемых скаффолдах на основе фиброина шелка в виде пленок. Подобное исследование для данных типов клеток с использованием технологии СЗОНТ выполнено впервые
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