Abstract
AimThe present work investigated serum levels of miR-29a, miR-122 and sestrin2 in obese children with/without type-2-diabetes mellitus (T2DM), and their correlations with inflammatory, metabolic and anthropometric parameters.MethodsThe study included 298 children, divided into: G1 (control, n = 136), G2 (obese without diabetes, n = 90) and G3 (obese with T2DM, n = 72). Metabolic and anthropometric parameters, miR-29a, miR-122 relative expressions, and sestrin2, high sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels were measured by their specific methods. The data was processed and analyzed by SPSS V.26 using the corresponding tests. After testing the variables’ normality, Kruskal–Wallis one-way-ANOVA, Spearman correlations coefficient were used.ResultsSignificant higher serum miR-29a, miR-122, IL-6, hsCRP and TNF-α and lower sestrin2 levels were found in G2 and G3 than G1 and in G3 than G2 (p= > 0.001 for all). Especially in G3, miR-29a and miR-122 levels correlated positively while sestrin2 levels correlated negatively with waist circumference and BMI percentiles, serum levels of LDL-cholesterol, triacylglycerol, total cholesterol, HbA1c%, glucose, insulin, c-peptide, homeostatic model assessment-insulin resistance (HOMA-IR), IL-6, hsCRP and TNF-α.ConclusionThe change in the serum miR-29a, miR-122 and sestrin2 levels in obese children with/without T2DM may suggest a possible role of these biomarkers in the pathogenesis of childhood obesity and their accompanied complications e.g. inflammations and T2DM. Also, further studies are required to test drugs that antagonize the action miR-29a and miR-122 or upregulate sestrin2 in the management of these cases.
Highlights
The prevalence of childhood and adolescence obesity has increased markedly in the last decade [1, 2]
Obesity is associated with dysregulation in the adipocyte-derived cytokines that leads to a generalized low inflammatory condition and insulin resistance [9, 10]
Comparison of different variables in the 3 studied groups The current study found significant high levels of miR29a, miR-122, IL-6, high sensitivity C-reactive protein (hsCRP) and tumor necrosis factor-α (TNF-α) in obese (G2) and obese with type 2 diabetes mellitus (T2DM) (G3) groups compared to the control (G1) group (Table 1) and in obese with T2DM (G3) group compared to obese (G2) group
Summary
The prevalence of childhood and adolescence obesity has increased markedly in the last decade [1, 2]. Obese children have a high risk of developing many cardiac and metabolic complications such as type 2 diabetes mellitus (T2DM) either during their childhood or, later during their adulthood [5, 6]. Both obesity and T2DM are considered as systemic inflammatory conditions [7, 8]. Obesity is associated with dysregulation in the adipocyte-derived cytokines that leads to a generalized low inflammatory condition and insulin resistance [9, 10]. Many previous studies were conducted to understand the pathogenesis of the obesity accompanied inflammations in order to prevent, control and manage them [7,8,9,10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
