Investigation of the level of DNA double-strand breaks and mechanisms of cell death under irradiation of lung cancer and melanoma cells with ultra-high dose rate photon radiation

Tm Kulinich,Yua Bykov,Am Shishkin,Eg Krastelev,Vk Bozhenko,Av Ivanov,Vp Smirnov

doi:10.24075/brsmu.2018.066

Abstract

Research into the effects of radiation delivered at ultrahigh dose rates > 1 × 107 Gy/min to biological objects is a new promising area of radiobiology. The unique characteristics of the high-current nanosecond electron accelerator Mir-M enable its use in medical and biological research, specifically in the experiments aimed at investigating the effect of therapeutic doses at a dose rate up to 100 MGy/s. In this work we study the effects of ultrahigh dose rate photon radiation on human lung carcinoma (A549) and melanoma (MelMtp-x) cells lines and compare them with those of the therapeutic gamma unit Rokus-AM. We show that ultrahigh dose rates induce more significant damage in the studied cell lines at doses between 2 and 7 Gy, radioresistant melanoma being more sensitive to photon radiation delivered at ultrahigh dose rates.

Highlights

Research into the effects of radiation delivered at ultrahigh dose rates > 1 × 107 Gy/min to biological objects is a new promising area of radiobiology
In this work we study the effects of ultrahigh dose rate photon radiation on human lung carcinoma (A549) and melanoma (MelMtp-x) cells lines and compare them with those of the therapeutic gamma unit Rokus-AM
We show that ultrahigh dose rates induce more significant damage in the studied cell lines at doses between 2 and 7 Gy, radioresistant melanoma being more sensitive to photon radiation delivered at ultrahigh dose rates

Summary

Introduction

Research into the effects of radiation delivered at ultrahigh dose rates > 1 × 107 Gy/min to biological objects is a new promising area of radiobiology. In this work we study the effects of ultrahigh dose rate photon radiation on human lung carcinoma (A549) and melanoma (MelMtp-x) cells lines and compare them with those of the therapeutic gamma unit Rokus-AM. We show that ultrahigh dose rates induce more significant damage in the studied cell lines at doses between 2 and 7 Gy, radioresistant melanoma being more sensitive to photon radiation delivered at ultrahigh dose rates. Целью работы было исследовать влияние фотонного излучения сверхвысокой мощности на клетки опухолевых линий рака легкого (А549) и меланомы (MelMtp-x), провести сравнение полученных эффектов с воздействием на клетки излучения терапевтической гаммаустановки «Рокус-АМ». The efficacy of treatment can be improved by applying ultrahigh dose rate radiation, which at the same time can reduce the adverse effects of radiotherapy

Objectives

Methods

Results

Conclusion