Investigation of the Laser Radiation Effect on the Viability and Functional Activity of Prokaryotic and Eukaryotic Cells

Abstract

Currently, laser technology is widely used in medicine. However, relatively few studies on the simultaneous effect of laser radiation with similar parameters on the viability and functional activity of prokaryotic and eukaryotic cells. When evaluating the effect of laser radiation, especially in a clinical setting, it is necessary to take into account its possible effect not only on cells of conditionally pathogenic microorganisms, but also on human cells. In this study, it was shown that laser radiation of a diode laser with a wavelength of 405 nm (blue) and 650 nm (red) has a pronounced bacteriostatic effect. Thus, red laser radiation with a power of 22 mV allows you to achieve a bacteriostatic effect against Staphylococcus aureus, but it significantly reduces the viability of buccal epithelial cells. The results can be considered on the one hand as a side effect of laser radiation, and on the other hand, the removal of dying cells filled with microorganisms can enhance the effect of the therapy.