Investigation of the kinetic properties of Phyllanthus chamaepeuce Ridl. extracts for the inhibition of pancreatic lipase activity

Abstract

The ethanolic extract from leaves of Phyllanthus chamaepeuce Ridl. was reported to have the ability to inhibit pancreatic lipase activity in vitro. In order to find the most potent of pancreatic lipase inhibitors from this plant, roots, barks, woods, and leaves which were extracted by using different solvents were used to investigate their ability to inhibit pancreatic lipase activity by in vitro test assay. The findings have shown that ethanolic and methanolic leaf extracts (at a concentration of 100 μg/mL) exhibited the greatest inhibition against porcine pancreatic lipase with 96.13 % (IC50: 2.98 ± 1.11 μg/mL) and 95.93 % (IC50: 4.36 μg/mL), respectively. Statistical analysis demonstrated strong positive significant correlations between the contents of phytochemical compounds (polyphenols, flavonoids, and alkaloids) and % pancreatic lipase inhibition. The result from enzyme kinetic assay indicated that ethanolic and methanolic leaf extracts from P. chamaepeuce Ridl. noncompetitively inhibited pancreatic lipase activity. These findings seem to indicate the use of P. chamaepeuce Ridl. extracts as an anti-obesity agent.