Abstract
Simple SummaryBroad-spectrum antibiotics have been a cornerstone in the treatment of bacterial diseases. However, growing evidence suggests that antibiotics have effects on host-associated gut microbiota communities. In this study, we report persistent significant changes in the abundance of gut microbiota and their functional metabolite pathways in chickens due to enrofloxacin and diclazuril exposure. These changes may affect the taxonomic, genomic, and functional capacity of the chicken gut microbiota, reducing bacterial diversity while expanding and collapsing membership of specific indigenous taxa. Understanding the biology of competitive exclusion of adaptive functions during antibiotic exposure in the gut may inform the design of new strategies to treat infections, while preserving the ecology of chicken-beneficial constituents.The dynamic microbiota in chickens can be affected by exposure to antibiotics, which may alter the composition and substrate availability of functional pathways. Here, 120 Jing Hong chicks at 30 days of age were randomly divided into four treatments totaling seven experimental groups: control chicks not exposed to antibiotics; and chicks exposed to enrofloxacin, diclazuril, and their mixture at 1:1 for 14 days and then not exposed for a withdrawal period of 15 days. Fecal samples were collected from the 7 groups at 8 time-points (exposure to 4 antibiotics and 4 withdrawal periods) to perform in-depth 16S rRNA sequencing of the gut microbiota. Taxon-independent analysis showed that the groups had significantly distinct microbial compositions (p < 0.01). Based on the microbial composition, as compared with the control group, the abundances of the phyla Firmicutes, Actinobacteria, Thermi, and Verrucomicrobia, as well as the families Lactobacillus, Lactococcus, S24-7, and Corynebacterium, were decreased in the antibiotic-exposed chicks (p < 0.01). Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analyses revealed significant differences in microbiota metabolite pathways due to the genera of the antibiotic-responsive microbes (p < 0.01), especially the pathways relating to cell growth and death, immune system diseases, carbohydrate metabolism, and nucleotide metabolism. Oral treatment with enrofloxacin, diclazuril, and their mixture modified the gut microbiota composition and the microbial metabolic profiles in chickens, with persistent effects (during the withdrawal period) that prevented the return to the original community and led to the formation of a new community.
Highlights
IntroductionAnticoccidial products are often used to control diseases such as coccidiosis in flocks of chickens.only a few antibiotic drugs (such as enrofloxacin and diclazuril) have great efficacy against pre-existing infections, such as Salmonella spp., Campylobacter, and Eimeria spp. [1,2]
Anticoccidial products are often used to control diseases such as coccidiosis in flocks of chickens.only a few antibiotic drugs have great efficacy against pre-existing infections, such as Salmonella spp., Campylobacter, and Eimeria spp. [1,2]
These drugs have been correlated with alterations in both the structure and function of gut microbiota, with temporary effects that return to the originating community and sometimes persist, forming a new community [27]
Summary
Anticoccidial products are often used to control diseases such as coccidiosis in flocks of chickens.only a few antibiotic drugs (such as enrofloxacin and diclazuril) have great efficacy against pre-existing infections, such as Salmonella spp., Campylobacter, and Eimeria spp. [1,2]. [1,2] These antibiotic affect the gut microbiota and their metabolic pathways [3,4]. Gut microbiota may alter the pathophysiology of parasite infections, and changes in microbiota can confer resistance to enteric protozoa or can promote protozoan infection, because normal or healthy microbiota decrease the host susceptibility to this parasite [6,7]. Host metabolomics may enable global metabolite perturbations in response to the antibiotics mediated from gut secretion. These antibiotics have been found to alter the structural, compositional, and functional capacity of gut microbiota in antibiotic-exposed hosts [8]. Antibiotic treatment in vitro and in vivo decreases the number of bacteria and the diversity of the microbiota from days to weeks after the cessation of antibiotic administration, it is possible that some bacterial species could be permanently depleted from the community [9,10]
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