Investigation of the Impact of the Addition of Probiotic Mixtures AQUA CLEAR-S® as Food Supplement on Hematology Parameters and Intestinal Histology of Red Tilapia (Oreochromis sp.)

Abstract

Background: The use of antibiotics in fish feed have been globally restricted due to having its huge negative consequences, dietary prebiotics, probiotics, symbiotics, phytobiotics and other functional dietary supplements are thought to be alternative substitutes. The goal of the study was to determine the effects of adding probiotic mixtures AQUA CLEAR-S® as a feed additive on red tilapia (Oreochromis sp.) intestinal histology and hematology parameters. Methods: The fish were given either the basic experimental nutrition (the control group) or one of three experimental diets that included the basic diet supplemented with 1, 2, or 3 g of AQUA CLEAR-S® for T1, T2 and, T3, respectively. The four experimental treatments were done in three replicates each. Twelve (50 × 40 × 60 cm) aquariums, each with ten fish (avg. wt. 6.00 g±0.17), were used. The studied parameters were basic hematological and immunological blood parameters, antioxidant activities and gut cytology. Result: Probiotic supplementation significantly improved the hematology parameters in red tilapia fingerlings after probiotic treatments. This trend was continued in the gut architecture. As a result, Oreochromis sp. had enhanced health when probiotics were incorporated into their diet. AQUA CLEAR-S®-treated fish also showed a noticeable change in their fish’s intestinal histology. The results of the study showed that consuming probiotics increased hematology parameters indicators and gut cytology of red tilapia.