Abstract
Background/aimThe aim of the study is to assess expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in tumors and peripheral bloods of colorectal cancer patients in stages I–IV.Materials and methodsThe mRNA levels of the genes were determined in tumor tissues and peripheral blood samples of 45 colorectal cancer patients and colon tissues and peripheral blood samples of 5 healthy individuals. Real-time polymerase chain reaction method was used for the analysis.ResultsThe mRNA level of the CPEB4 gene was significantly downregulated in colorectal tumor tissues and was upregulated in the peripheral blood of colorectal cancer patients relative to the controls (P < 0.05). APC mRNA level was significantly downregulated in tissues and upregulated in the peripheral blood (P < 0.05). TRIP13 mRNA level was upregulated in peripheral blood and also significantly upregulated in colorectal tumor tissues (P < 0.05). EIF2S3 mRNA level was upregulated in tissues and also significantly upregulated in peripheral blood (P < 0.05). PIK3CA mRNA level was downregulated in tissues and upregulated in peripheral blood. EIF4A1 mRNA level was downregulated in tissues and significantly upregulated in peripheral blood (P < 0.05). CTNNB1 mRNA level was downregulated in tissues and upregulated in peripheral blood. IFNg mRNA level was upregulated in both colorectal cancer tumor tissues and peripheral blood.Conclusion:TRIP13 and CPEB4 mRNA up regulation in the peripheral blood of patients with colorectal cancer may be a potential target for early stage diagnosis. In addition to this evaluation, although there is not much study on EIF2S3 and EIF4A1 mRNA changes in cases with colorectal cancer, upregulation in peripheral blood draws attention in our study. These data will shed light on the new comprehensive studies.
Highlights
Cancers originating from the colon or rectum are called colorectal cancer
TRIP13 and CPEB4 mRNA up regulation in the peripheral blood of patients with colorectal cancer may be a potential target for early stage diagnosis
Gene expression analysis The mRNA levels of CPEB4, adenomatous polyposis coli (APC), TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes expressed in colorectal cancer tissue specimens, colorectal cancer blood samples, normal colon tissues and blood samples were analysed
Summary
Cancers originating from the colon or rectum are called colorectal cancer. Colorectal cancer is the third most common cancer type in the world and the fourth most common cause of cancer-related deaths [1]. Several studies have been conducted to investigate the difference in gene expression levels between tumor and normal colorectal tissues and have reported significant differences in gene expression profiles between adenoma and normal mucosa. Among these studies, certain groups of genes have been reported to be of differently expressed and help distinguish cancerous tissues from normal ones [4,5,6,7,8,9,10]. Mutations in APC gene are the basis of hereditary predisposition to colorectal cancer in familial adenomatous polyposis coli
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