Abstract
Here, we report the development of a new polyacrylamide (PAAm)/starch nanofibers’ blend system and highlight its potential as substrate for efficient enzyme immobilization. PAAm was synthesized and blended with starch. The final blend was then electrospun into nanofibers. The response surface methodology was used to analyze the parameters that control nanofiber’s diameter. Electrospun mat was then modified either by cross-linking or phytase immobilization using silane coupling agent and glutaraldehyde chemistry. Physico-chemical properties of blends were investigated using spectroscopic and thermal studies. The evaluation of immobilized enzyme kinetics on both pure and the starch blended PAAm nanofibers was performed using Michaelis–Menten kinetic curves. Fourier transform infrared spectroscopy results along with differential scanning and X-ray diffraction confirmed that blending was successfully accomplished. TGA analysis also demonstrated that the presence of starch enhances the thermal degradability of PAAm nanofibers. Finally, it was shown that addition of starch to PAAm increases the efficacies of enzyme loading and, therefore, significantly enhances the activity as well as kinetics of the immobilized enzyme on electrospun blend mats.
Highlights
There has been a great progress in the preparation, function, and evaluation of biomaterials concerning multifaceted practicability of them
The scanning electron microscopy (SEM) images and Response surface methodology (RSM) diameter analysis have shown that more percentage of starch addition—proportionally against the percentage of PAAm—could lead to the reduction of the resultant nanofibers’ diameter
The thermal properties of PAAm nanofibers were shown to be improved by the presence of starch which enhanced the thermal degradability of PAAm nanofibers
Summary
There has been a great progress in the preparation, function, and evaluation of biomaterials concerning multifaceted practicability of them. Thanks to the outstanding properties and the key role in diversified areas of performance, a wide range of biomaterials has been increasingly used in biological applications. The advantage that glycosidic bonds between monosaccharides provide polysaccharides with good biocompatibility and availability properties makes it suitable to be used as cell carriers in tissue engineering It was observed that various blends of polysaccharide component show good potential to be used in numerous biomedical applications (Shelke et al 2014). Polysaccharides such as starch modified nanofibrous scaffolds are capable of promoting cellular activities through increasing bioactivity and cell retention Biocompatibility and cell cultural properties of scaffolds obtained from blended starch nanofibers can significantly intensify the cells
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