Investigation of the effects of p-Coumaric acid on MDA, some antioxidants and histopathological parameters in nephrotoxicity induced by bisphenol A in rats

Abstract

The aim of this study was to investigate p-Coumaric acid (PCA) as a protector in detecting oxidative and targeted damage in the elongation tissue of rats induced by bisphenol A (BPA). BPA is an environmental pollutant widely used in many areas. The widespread use of this substance increases its transmission to humans. BPA causes damage to many tissues and organs. One of the most frequently damaged organs is the kidney. In this study, fifty healthy male rats were used to determine the effects of BPA and PCA. A total of five experimental groups were formed with ten animals in each group. Group 1 was set as the Control, Group 2 as BPA, Group 3 as BPA+PCA50, Group 4 as BPA+PCA100, and Group 5 as PCA100. The experiment lasted 14 days. Hematoxylin eosin staining and ELISA analyses were performed on kidney tissues at the end of the experiment. Malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and nitric oxide (NO) levels were determined in the kidney tissues by ELISA analysis. It was observed that kidney MDA and NO levels increased in the groups administered BPA while SOD and GSH levels decreased. Histopathologically, degeneration and necrosis in the tubular epithelium, glomerular atrophy, and hyperemia in the vessels were observed in the kidney tissues of the BPA groups. In the BPA+PCA groups it was seen that PCA prevented the negative effects of BPA in a dose-dependent manner, and helped in the maintenance of values close to the control group.