Abstract
Objective To investigate the effect induced by specific inducible nitric oxide synthase (iNOS) inhibitor 1 400 W on nasopharyngeal carcinoma CNE-2 cells lines and its mechanism. Methods CNE-2 cells were treated by different concentrations of 1 400 W, diamminedichloroplatinum (DDP), and both chemicals. Cell counting kit-8 (CCK-8) was used to examine the viability of cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect the iNOS mRNA expression. Results The expression of iNOS mRNA was down-regulated by 1400W and was positively correlated with inhibition rate of cell proliferation. 1 400 W inhibits proliferation of CNE-2 cell in a concentration-dependent manner. The proliferation inhibition rate of CNE-2 cells treated by 1 400 W combined with DDP was not enhanced. Conclusions Specific inducible nitric oxide synthase inhibitor 1 400 W can exerts anti-tumor effect though inhibiting the expression of iNOS mRNA; The mechanism of chemosensitization induced by iNOS inhibitor on CNE-2 cells may be closely related level of down-regulation of iNOS expression. Key words: Nitric oxide synthase/AI/ME; Nasopharyngeal neoplasms/DT/ME
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