Investigation of the effect of repeated use of PRIVD on serum progesterone, estrogen levels, and ovulatory follicle diameter in pubertal heifers

Armağan Çolak,Mehmet Cengi̇z,Muhammad Furqan Asghar Chacher

doi:10.3906/vet-1809-46

Abstract

The purpose of this study was to evaluate the effect of reuse of progesterone-releasing intravaginal devices (PRIVDs) on serum progesterone, estrogen levels, and ovulatory follicle diameter in pubertal heifers. Three pubertal Holstein heifers were used in the study for three different periods with the usage of three PRIVDs up to three times from day 0 to 7 according to the Latin square model. Blood samples were collected on days 0, 3, 5, 7, and 9 for hormonal analysis. Ovulatory follicle diameter was measured by transrectal ultrasonography 60 h after removal of the PRIVD. Serum progesterone demonstrated a significant relationship with device use and day (P 0.05) and ovulatory follicle diameter (P > 0.05) did not differ significantly with the use of the PRIVD at day 9. In the present study, even the 3rd use of the PRIVD produced similar results as the first use. In conclusion, PRIVDs can be reused or manufactured with low progesterone for heifers to reduce hormonal synchronization cost.

Highlights

Continuous expansion in herd size of the dairy industry brings forth the need of development of systematic programs for reproductive management of dairy cattle
Mean serum progesterone concentration decreased with each use of the progesterone-releasing intravaginal devices (PRIVDs) as expected and maximum progesterone was seen in the 1st use
There was no significant difference between mean serum progesterone levels of the 2nd and 3rd uses

Summary

Introduction

Continuous expansion in herd size of the dairy industry brings forth the need of development of systematic programs for reproductive management of dairy cattle These programs have markedly evolved in the past 20 years with synchronization protocols, which allow the use of fixed-time artificial insemination (FTAI). Estrous synchronization with the use of FTAI programs has become an integral part of present-day reproductive management, which reduces the time and labor for estrus detection and increases the profitability of dairy farms [1,2] Progestins such as melengestrol acetate, controlled internal drug release (CIDR®), the progesterone-releasing intravaginal device (PRID Delta®), and norgestomet implants (Syncro-mate B®) are being used very often in FTAI-based synchronization protocols [3,4]. The progesterone in a PRIVD suppresses the release of LH and ovulation at introduction and releases LH, supports follicle

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Discussion

Conclusion