Investigation of the effect of chitosan and silver nanoparticles on the antibiotic resistance of Escherichia coliO157:H7 isolated from some milk products and diarrheal patients in Sohag city, Egypt.

Alshimaa A Hassanien,Eman M Shaker

doi:10.14202/vetworld.2020.1647-1653

Alshimaa A Hassanien, Eman M Shaker

Open Access

https://doi.org/10.14202/vetworld.2020.1647-1653

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Journal: Veterinary world	Publication Date: Jan 1, 2020
Citations: 5	License type: CC BY 4.0

Affiliation: Sohag University

Abstract

Background and Aim:Antimicrobial-resistant Escherichia coli O157:H7 causes serious diseases in humans, especially when circulated in their food. This study was designed to detect the presence of E. coli O157:H7 using the fliC H7 gene in some milk products as kareish cheese, labena, and yoghurt sold in Sohag city, Egypt, and among diarrheal patients admitted to governmental hospitals in Sohag and also to highlight the risk factors associated with their infection. In addition, the antimicrobial resistance and the effect of chitosan nanoparticles (CNP) and silver nanoparticles (SNP) on E. coli O157:H7 isolates obtained from both milk products and patients were investigated.Materials and Methods:Microbiological culture methods and polymerase chain reaction were used for detecting E. coli O157:H7 in 150 milk products and 150 stool samples. Resistance against some antimicrobials that were used in the treatment of animals and humans was investigated using the disk diffusion technique. CNP and SNP at two concentrations (30 and 60 μg/mL) and average sizes of 25.1 and 26.5 nm, respectively, were identified by transmission electron microscopy. Their effect on E. coli O157:H7 isolates was examined using the well diffusion method. Risk factors for infection were investigated using statistical analysis.Results:There were 11.3% and 14.7% of milk products and stool samples positive for E. coli O157:H7, respectively. These isolates exhibited high antimicrobial resistance to ampicillin, tetracycline, and gentamycin. CNP and SNP demonstrated inhibitory effects on E. coli O157:H7 growth, which significantly increased at high concentrations (60 μg/mL), with mean inhibition zones of 31.941±3.749 and 30.681±3.871 mm for CNP in milk products and patient isolates, respectively. The respective values for SNP were 33.588±3.675 mm and 32.500±2.444 mm, indicating a higher bactericidal effect than that of CNP. Regarding risk factors for infection, both young and elderly subjects and those in contact with infected persons and/or having chronic diseases were infected.Conclusion:CNP and SNP are suitable for both medical and agricultural applications for disease control and enhancement of food quality.

Highlights

Escherichia coli O157:H7 is considered as a serotype of Shiga toxin-producing E. coli that is transmitted through the fecal-oral route, primarily through food, especially of animal origin, in the form of undercooked meat and raw milk, and secondarily from person to person
chitosan nanoparticles (CNP) and silver nanoparticles (SNP) demonstrated inhibitory effects on E. coli O157:H7 growth, which significantly increased at high concentrations (60 μg/mL), with mean inhibition zones of 31.941±3.749 and 30.681±3.871 mm for CNP in milk products and patient isolates, respectively
This study was designed to detect the presence of E. coli O157:H7 using the fliC H7 gene in some milk products as kareish cheese, labena, and yoghurt sold in Sohag city, Egypt, and among diarrheal patients admitted to governmental hospitals in Sohag and to highlight the risk factors associated with their infection

Summary

Introduction

Escherichia coli O157:H7 is considered as a serotype of Shiga toxin-producing E. coli that is transmitted through the fecal-oral route, primarily through food, especially of animal origin, in the form of undercooked meat and raw milk, and secondarily from person to person. It causes gastrointestinal sickness ranging from watery diarrhea and abdominal cramps to hemorrhagic colitis, hemolytic uremic syndrome, and renal failure. Antimicrobial-resistant Escherichia coli O157:H7 causes serious diseases in humans, especially when circulated in their food. The antimicrobial resistance and the effect of chitosan nanoparticles (CNP) and silver nanoparticles (SNP) on E. coli O157:H7 isolates obtained from both milk products and patients were investigated

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