Investigation of the Callus Induction Medium and the Regeneration Medium in Rice Anther Culture.

Abstract

We determined the callus induction medium and the regeneration medium to efficiently utilize the anther culture technique in rice breeding. The medium containing N6 basal medium supplemented with 4 mg L-1 of 2, 4-D effectively induced calli from microspores of some japonica varieties and two F1 hybrids with japonica varieties. The regenerated plants containing green and/or albino shoots were produced efficiently from microspore-derived calli on the regeneration medium containing LS basal medium supplemented with sorbitol (30000 mg L-1), CH (2000 mg L-1), MES (1000 mg L-1), NAA (2 mg L-1), and kinetin (0.5 mg L-1) solidified by gellun gum (4000 mg L-1).