Investigation of the Blood-Ganglion Barrier Properties in Rat Sympathetic Ganglia by Using Lanthanum Ion and Horseradish Peroxidase as Tracers

Abstract

Vascular permeability in various rat sympathetic ganglia, including superior cervical ganglia, thoracic ganglia and the celiac-mesenteric ganglia (CMG) complex, was investigated by using lanthanum and horseradish peroxidase (HRP) as tracers with special attention to the neuronal and small granule-containing (SGC) cell area. After lanthanum perfusion, lanthanum tracer was present within the lumen of blood vessels. No lanthanum depositions were found in the extravascular space surrounding neurons in the superior cervical and thoracic ganglia. By contrast, an accumulation of lanthanum was observed in both luminal, abluminal and subendothelial surface of blood vessels in neuronal and SGC cell areas of the CMG complex and surrounding SGC cells in superior cervical ganglia. Injecting HRP revealed that all blood vessels of various sympathetic ganglia, either in neuronal or in SGC cell areas, were impermeable to HRP. HRP reaction product was limited to the vascular lumen and macrophages. The escape of HRP was obstructed by the junctional complex at intercellular clefts of endothelia and also by the diaphragms of the fenestrated capillaries associated with SGC cells. We conclude that there are different properties in the blood-ganglion barriers among rat sympathetic ganglia: (1) continuous capillaries in superior cervical ganglia and thoracic ganglia provide an efficient blood-ganglion barrier that prevents the penetration of tracers, and (2) capillaries in the CMG complex and in regions of the superior cervical ganglia that contain SGC cells possess a selective blood-ganglion barrier that discriminates between tracers based on their molecular sizes.