Investigation of the Association Between 584C/T Polymorphism of EL Gene and Risk of Premature Coronary Artery Disease in Fars Province.

Samane Toosi,Zeinab Ahmadi,Salma Radmanesh,Sara Senemar

doi:10.15171/jcvtr.2015.25

Abstract

Introduction: Endothelial lipase (EL) is a protein from the triglyceride lipase family which plays an important role in high-density lipoprotein (HDL) metabolism. One of the most frequently studied variants is 584C/T which causes the amino acid threonine at codon 111 to convert to isoleucine. Many studies have shown the association of this variant with HDL-C level and CAD disease. Methods: The population of this study consists of 140 patients (all males) with angiographically confirmed coronary artery disease (CAD) and 80 controls. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was carried out for genotyping of LIPG 584C/T. Data were analyzed using SPSS. Results: The results of the study indicated that the frequency of T allele was significantly lower among CAD patients than among controls (0.27 vs 0.36, P = .004). However, no significant correlation was found between the 584C/T variant and serum HDL-C level. Multivariate regression analysis confirmed that the T allele is significantly associated with CAD disregarding the age, hypertension, hypercholesterolemia, diabetes and HDL-C (OR = 0.494, 95% CI = 0.253- 0.968, P =.040). Conclusion: It was concluded that the T allele was associated with protection from CAD in Fars province independent of HDL-C level.

Highlights

Endothelial lipase (EL) is a protein from the triglyceride lipase family which plays an important role in high-density lipoprotein (HDL) metabolism
One of the most important risk factors for coronary artery disease (CAD) is dyslipidemia which is comprised of high levels of plasma total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL) and low levels of high-density lipoprotein (HDL)
584C/T is the most frequently studied variant that can alter the amino acid threonine, converting into isoleucine at codon 111.12,13 Some studies supported the idea that the 584C/T variant was inversely correlated with serum high-density lipoprotein-cholesterol (HDL-C) levels[14,15] but in other studies this association was not found.[11,16]

Summary

Introduction

Endothelial lipase (EL) is a protein from the triglyceride lipase family which plays an important role in high-density lipoprotein (HDL) metabolism. HDL-C levels are inversely correlated with CAD.[1,2,3] High HDL-C levels are associated with the protection from CAD and low HDL-C levels are related with elevated risk of CAD.[4] Endothelial lipase (EL) is a protein from the triglyceride lipase family which is expressed in the endothelial cells and tissues like the liver, lung, macrophage, testis, ovary and placenta. Several genetic variants have been recognized in the LIPG gene.[4] Among these, 584C/T is the most frequently studied variant that can alter the amino acid threonine, converting into isoleucine at codon 111.12,13 Some studies supported the idea that the 584C/T variant was inversely correlated with serum HDL-C levels[14,15] but in other studies this association was not found.[11,16] So far, the correlation between 584C/T variant and the risk of CAD and HDL-C level has not been established in Fars province. In the present study the effect of 584C/T variant on the risk of CAD and HDL-C levels was investigated in Fars province

Methods

Results

Conclusion