Abstract
BackgroundThe digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.MethodsWe attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities.ResultsThe inhibition of lymphoblastoid cell proliferation was noted from 8.5 μg/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner.ConclusionIn summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent.
Highlights
The digallic acid (DGA) purified from Pistacia lentiscus
Evaluation of antiproliferative activity Human lymphoblastoid TK6 cells were treated with the compound isolated from P. lentiscus fruits, for 48 h at 37°C
Induction of apoptotic DNA fragmentation by DGA The fragmentation of TK6 cell DNA was detected on a 1.5% agarose gel electrophoresis after exposing 1.5 x 106 cells to 0, 2.5, 5 and 10 μg/ml of DGA during 24 h and 48 h
Summary
The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Polyphenols were described to be beneficial against human diseases such as cancer and metastasis [1,2] It has been shown, in both in vitro test and small animal model study, that polyphenols induce responses consistent with the protective effects of diets rich in fruits and vegetables against degenerative conditions such as cardiovascular disease and carcinogenesis [3,4]. In both in vitro test and small animal model study, that polyphenols induce responses consistent with the protective effects of diets rich in fruits and vegetables against degenerative conditions such as cardiovascular disease and carcinogenesis [3,4]
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