Investigation of the antioxidant effects of pheniramine maleate and nebivolol on testicular damage in rats with experimentally induced testis torsion.

Ercan Yuvanc,Mahi Balci,Ucler Kisa,Tunc Ozan,Devrim Tuglu,Erdal Yilmaz,Ertan Batislam

doi:10.1590/s0102-865020180020000004

Ercan Yuvanc, Mahi Balci + Show 5 more

Open Access

https://doi.org/10.1590/s0102-865020180020000004

Copy DOI

Abstract

To investigate the biochemical, histopathologic, and spermatogenetic changes in the detorsionated testicle after experimental torsion and to study the antioxidant effects of pheniramine maleate and nebivolol. Twenty-four Sprague-Dawley male rats were divided into 4 groups: Group 1: Sham; Group 2: Torsion/Detorsion (T/D); Group 3: T/D + Pheniramine maleate (PM); Group 4: T/D + Nebivolol (NB) group. Paroxanase (PON), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stres index (OSI) were measured, and spermatogenetic and histopathologic evaluation was performed in tissue and blood samples. The evaluation of tissue TAS indicated no statistically significant difference in Group 3 compared to Group 2. A statistically significant increase was detected in Group 4 compared to Group 2. Serum PON levels revealed a statistically significant increase in Groups 3 and 4 compared to Groups 1 and 2. The Johnsen testicular biopsy score decreased in Groups 3 and 4, but the decrease was not statistically significant. Pheniramine maleate and nebivolol have antioxidant effects against ischemia-reperfusion damage. They also support tissue recovery, which is more significantly observed by nebivolol.

Highlights

An acute scrotum is a pediatric urologic emergency and commonly presentesas the torsion of the testis or appendix testis
Testicular torsion occurs due to rotation of the spermatic cord and its accessories because of the anastomosis free terminal testicle artery and the nonelastic structure of the tunica albuginea, leading to the blockage of the arterial and venous blood flow
Besides the blockage of testicular blood flow, ischemia reperfusion (I/R) damage developing due to the defense system of the testicle plays an important role in the formation of atrophy and loss of testicular functions

Summary

Introduction

An acute scrotum is a pediatric urologic emergency and commonly presentesas the torsion of the testis or appendix testis. Testicular torsion occurs due to rotation of the spermatic cord and its accessories because of the anastomosis free terminal testicle artery and the nonelastic structure of the tunica albuginea, leading to the blockage of the arterial and venous blood flow. Besides the blockage of testicular blood flow, ischemia reperfusion (I/R) damage developing due to the defense system of the testicle plays an important role in the formation of atrophy and loss of testicular functions. Pheniramine maleate (PM) is an alcylamine-derived H1 antihistaminic, mostly used for the symptomatic treatment of allergies and the flu due to its high agregation capacity to cell membranes because of its lipophilic features and resulting prevention of the formation and/or secretion of free radicals and its anti-inflammatory effect[1]. Its long-term use has been shown to support endothel function, to prevent the formation of vascular super oxide by producing NADPH oxidase, and to supress vascular oxidative stress in antihypertensive treatment doses[2,3]

Methods

Results

Conclusion