Abstract
Novel synthesized Chitosan–Copper oxide nanocomposite (Cs–CuO) was prepared using pomegranate peels extract as green precipitating agents to improve the biological activity of Cs-NP's, which was synthesized through the ionic gelation method. The characterization of biogenic nanoparticles Cs-NP's and Cs–CuO-NP's was investigated structurally, morphologically to determine all the significant characters of those nanoparticles. Antimicrobial activity was tested for both Cs-NP's and Cs–CuO-NP's via minimum inhibition concentration and zone analysis against fungus, gram-positive and gram-negative. The antimicrobial test results showed high sensitivity of Cs–CuO-NP's to all microorganisms tested in a concentration less than 20,000 mg/L, while the sensitivity of Cs-NP's against all microorganisms under the test started from a concentration of 20,000–40,000 mg/L except for the C. albicans species. The hematological activity was also tested via measuring the RBCs, platelet count, and clotting time against healthy, diabetic, and hypercholesteremia blood samples. The measurement showed a decrease in RBCs and platelet count by adding Cs-NP’s or Cs–CuO-NP's to the three blood samples. Cs-NP's success in decreasing the clotting time for healthy and diabetic blood acting as a procoagulant agent while adding biogenic CuO-NP’s to Cs-NP’s increased clotting time considering as an anti-coagulant agent for hypercholesteremia blood samples.
Highlights
Novel synthesized Chitosan–Copper oxide nanocomposite (Cs–CuO) was prepared using pomegranate peels extract as green precipitating agents to improve the biological activity of chitosan nanoparticles (Cs-NP’s), which was synthesized through the ionic gelation method
The main points concluded from this work could be summarized as the following, Point(I) the characterization of biogenic synthesized Cs–CuO-NP’s and Cs-NP’s found that both nanoparticles are in a spherical shape with particles size around 20–40 nm
The characterization provides Cs–CuO-NP’s formation as hybrid nano–composite from nanochitosan and copper oxide capped with extract of pomegranate peel
Summary
Novel synthesized Chitosan–Copper oxide nanocomposite (Cs–CuO) was prepared using pomegranate peels extract as green precipitating agents to improve the biological activity of Cs-NP’s, which was synthesized through the ionic gelation method. The measurement showed a decrease in RBCs and platelet count by adding Cs-NP’s or Cs–CuO-NP’s to the three blood samples. Chitosan plays a vital role in the regeneration of the wounded area via fibroblast proliferation and glucosamine presence, enhancing the earlier synthesis of hyaluronic acid to accelerate the healing process with minimal s carring[7]. It helps in revascularization and plays a role in protecting against a therosclerosis[8]. Copper is one of the metallic nanoparticles, which is a vital element, in trace amounts, that facilitates various enzymes, and it helps in skin regeneration, wound healing process, and a ngiogenesis[11]. The chemical reduction method is a facile process for synthesizing NP’s using biopolymeric m aterials[16] in achieving a better substantial bacteriostatic/bactericidal property
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