Investigation of surfactant protein-C gene on respiratory distress syndrome in the Han nationality newborns of the Inner Mongolia

Abstract

Objective To investigate the relationship between single nucleotide polymorphisms of surfactant protein C(SP-C) gene and respiratory distress syndrome(RDS) in the Han nationality newborns in Inner Mongolia and whether there is a mutation occurs on SP-C gene exon 4 and 5. Methods One hundred newborns with RDS(case group) and 100 newborns without RDS(control group) were selected.PCR gene analysis was used to establish the genotype and allele frequencies of exon 4(T138N) and 5(S186N) on SP-C. Results In the Han nationality newborns of Inner Mongolia region, there was no mutation on SP-C gene exon 4 and 5.Exon 4(T138N) on SP-C could be checked out three genotypes: namely AA, AC and CC.The genetic polymorphisms of exon 4 on SP-C were not statistically different between the case group and the control group(χ2=0.744, P=0.689). Besides, exon 5(S186N) on SP-C could also be checked out three genotypes: namely AA, AG and GG.The genetic polymorphisms of exon 5 on SP-C were also not statistically different between the case group and the control group(χ2=0.770, P=0.681). Conclusion There is no mutation on SP-C gene exon 4 and 5.The genetic polymorphism of exon 4 and 5 on SP-C displays no significant correlation with RDS of the Han nationality newborns in Inner Mongolia. Key words: Respiratory distress syndrome; Surfactant protein C; Genetic polymorphisms; Newborn