Abstract
The subcellular localization of several phosphoproteins that appear during the process of transformation of immature pollen to embryogenic cells was examined in tobacco. Analysis by fractionation of organelles on sucrose density gradients revealed that all of the phosphoproteins were present mainly in fractions with a density of 1.15 to 1.20 g · mL-1, and that they were localized in neither nuclei, plastids, microbodies, mitochondria, endoplasmic reticulum nor Golgi bodies. Appropriate levels of activity of marker enzymes of plasma membranes were not detected in the embryogenic cells, probably due to a specific physiological state.
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