Investigation of Soil Contamination With Cryptosporidium spp. Oocysts in Different Regions of Yazd, Central Iran

Abstract

Background: Cryptosporidium species are coccidian parasites that cause gastrointestinal disorders in humans and other animals worldwide. Objective: The aim of this study was to demonstrate the rate of contamination with Cryptosporidium spp. oocysts in soils collected from public parks, primary schools, green areas, kindergartens, suburban areas, streets, residential complexes, backyards and a passenger terminal in Yazd, central Iran. Materials and Methods: This cross-sectional study was conducted from September 2014 to February 2015, and the samples were collected from 9 different study sites and 56 regions. Soil samples were investigated by flotation technique and modified Ziehl–Neelsen staining for Cryptosporidium spp. oocysts. Finally, the slides were examined with a light microscope. The data were analyzed using SPSS software version 20.0 and chi-square statistical test. Results: Of a total of 220 soil samples, 47 (21.36%) were found to contain Cryptosporidium spp. oocysts. Statistical analysis showed that there was no significant difference between the contamination rate and different study sites in Yazd, central Iran (P>0.05). The highest contamination rate was observed in public parks (38.3%) and the lowest in passenger terminal, kindergartens and streets (4.25%) (P=0.934). Conclusion: The results of the present study show that the contamination of soil with Cryptosporidium spp. can be considered a serious problem in Yazd, central Iran. It should be considered particularly in public parks.