Investigation of sod2 gene polymorphism in the patients associated with type-2 diabetes mellitus patients

Abstract

Introduction: Type-2 Diabetes mellitus (type-2DM) is a multifactorial metabolic disorder, characterized by hyperglycemia. Oxidative stress plays a crucial role in the progression of diabetes and its complications. The polymorphism in the SOD2 gene (superoxide dismutase) results in minimal production of SOD enzymes, which may affect the scavenging of free radicals. Aim: to see the level of SOD enzyme in type-2DM patients associated with SOD2 gene polymorphism. Material& Methods: It is a comparative study conducted in the department of anatomy in collaboration with the Department of General Medicine, RKDF medical college Hospital& research center, Bhopal. 5 ml of venous blood from type-2 DM patients (n=30) and control subject (n=30) was collected, followed by estimation of blood glucose (HbA1c, FBS& PPBS) and the genetic test was done to detect the SOD2 gene polymorphism. Results: Blood glucose levels (HbA1c, FBS &PPBS) were high and SOD levels were low in type-2 DM patients when compared with control subjects (p<0.05). When a comparison was done among three genotypes, the levels of SOD enzyme were high in the CC genotype followed by CT and TT genotypes.