INVESTIGATION OF SEVERAL FACTORS ON ENZYMATIC HYDROLYSIS OF SUGAR BEET PULP AND CORN COB: STATISTICAL ANALYSES OF THE EXPERIMENTAL RESULTS

Abstract

In this work, sugar beet pulp (SBP) as a lignin poor biomass and corn cob (CC) as a lignin rich biomass were subjected to enzymatic hydrolysis to see the effects of various variables on reducing sugar yield. In SBP hydrolysis, response surface methodology (RSM) and ANOVA were used to fit sugar yield and to determine significance of the parameters (substrate, pectinase, cellulase and hydrolysis time). The proposed quadratic model gave an adequate approximation indicating the significance of all main effects and some of the interaction effects (p < 0.05). The maximum yields within the design space were found approximately as 87 g/L after 18 h of hydrolysis, using 300 µl Cellic Ctec3 and 300 µl Pectinex Ultra SP-L at %20 substrate loading. In CC hydrolysis, the use of nonionic surfactants (Tween 20 and Tween 80) under unpretreated conditions did not necessarily increase the yield of reducing sugar from untreated CC.