Abstract
Diphosphate compounds (KYP2O7) co-doped with Yb3+ and Er3+ ions were obtained by one step urea assisted combustion synthesis. The experimental parameters of synthesis were optimized using an experimental design approach related to co-dopants concentration and heattreatment as well as annealing time. The obtained materials were studied with theinitial requirements showing appropriate morphological (X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM)) and spectroscopic properties (emission, luminescence kinetics). Moreover, the effect of Er3+ and Yb3+ ions doped KYP2O7 on morphology, proliferative and metabolic activity and apoptosis in MC3T3-E1 osteoblast cell line and 4B12osteoclasts cell line was investigated. Furthermore, the expression of the common pro-osteogenic markers in MC3T3-E1 osteoblast as well as osteoclastogenesis related markers in 4B12 osteoclasts was evaluated. The extensive in vitro studies showed that KYP2O7 doped with 1 mol% Er3+ and 20 mol% Yb3+ ions positively affected the MC3T3-E1 and 4B12 cells activity without triggering their apoptosis. Moreover, it was shown that an activation of mTOR and Pi3k signaling pathways with 1 mol% Er3+, 20 mol% Yb3+: KYP2O7 can promote the MC3T3-E1 cells expression of late osteogenic markers including RUNX and BMP-2. The obtained data shed a promising light for KYP2O7 doped with Er3+ and Yb3+ ions as a potential factors improving bone fracture healing as well as in bioimaging (so-called in theranostics).
Highlights
In recent years, much attention has been paid to rare earth phosphate phosphors due to their appealing features, such as chemical stability and diversity in crystallographic structure [1]
We showed that KYP2O7 doped with 1 mol% of Er3+ and 20 mol% Yb3+in dose 500 μg/mL promotes osteoblasts metabolic activity and induces their highest proliferative potential
The most intense emission was obtained for samples co-doped with 1 mol% Er3+ and 20 mol% Yb3+ ions
Summary
Much attention has been paid to rare earth phosphate phosphors due to their appealing features, such as chemical stability and diversity in crystallographic structure [1]. The phosphates could be used as a matrix for doping with optically active ions, such as the rare earth metals. Potential application of the rare earth phosphates could be related to such areas as: cell bioimaging [2,3,4], light-emitting diodes [5,6,7,8], solar cells [9,10,11] as well as regenerative medicine. Potassium yttrium(III) diphosphate(V) KYP2O7 is a polymorphic compound. On the basis of ionic radius ratio (rK+/rY3+ = 1.68) value, polymorphism of KYP2O7 can be explained [12]. According to our knowledge the modern luminescent material KYP2O7 has never been employed as a matrix for investigation of up-conversion processes in biomedical applications
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
