Investigation of Promotion of Bone Matrices in Cultured Osteoblasts by Mechanical Vibration

Abstract

This paper describes effects of mechanical vibration on osteoblasts. Their bone mass generation was investigated when sinusoidal inertia force was applied to the cells. After the cells were cultured in culture plates in a CO2 incubator for one day and adhered on the cultured plane, vibration group of the culture plate was set on an aluminum plate attached to a exciter and cultured under sinusoidal excitation of 0.5 G and 25 Hz for 24 hours a day in another incubator separated from non-vibration group during 28 days of culture. Gene expression of alkaline phosphatase (ALP) was measured by a real-time reverse transcription polymerase chain reaction method. ALP activity was measured by Azo-dye method. Calcium salts generated by the cells were observed by being stained with alizarin red S solution. As a result, it is found that the mechanical vibration accelerates the gene expression and protein generation of ALP, and the calcium salt generation.