Investigation of PRL-RsaI and HaeIII gene polymorphisms in Anatolian water buffaloes bred by using PCR-RFLP method

Yusuf Özşensoy

doi:10.1590/rbz4720170166

Abstract

ABSTRACT The objective of this study was to investigate polymorphisms both in exons 1 and 3 of prolactin (PRL) gene for milk productivity of Anatolian water buffalo breed in Sivas province in Turkey. Blood samples were collected from 129 male and female water buffaloes and DNA was isolated by using phenol/chloroform method. Samples of DNA were amplified and resulting PCR products were digested with RsaI (for exons 1 and 3) and HaeIII (for exon 1). Allelic polymorphisms were determined by [...]

Highlights

Water buffaloes are raised in various countries for their economic value and as sources of workforce, meat, milk, leather, and horn (Michelizzi et al, 2010)
In another study conducted with Murrah water buffalo breed, RsaI restriction enzyme (RE) digestion of PRL exon 3 resulted in no fragments and only monomorphic AA genotype was obtained, which was considered as breed characteristic (Biradar et al, 2014)
Since Anatolian water buffalo breed is mainly raised for its milk, this study focused upon PRL gene and both exons 1 and 3 were investigated by using high amounts of samples

Summary

Introduction

Water buffaloes are raised in various countries for their economic value and as sources of workforce, meat, milk, leather, and horn (Michelizzi et al, 2010). RsaI restriction enzyme (RE) digestion in exon 3 of PRL resulted in two allelic polymorphisms (B and b) associated with milk productivity (Freeman et al, 2000; Alipanah et al, 2007). According to a study carried out with water buffaloes of both Murrah and Nili-Ravi breeds, results showed that A_ allele has the highest frequency (0.93 and 0.84, respectively) and no BB genotype was obtained (Mitra et al, 1995). In another study conducted with Murrah water buffalo breed, RsaI RE digestion of PRL exon 3 resulted in no fragments and only monomorphic AA genotype was obtained, which was considered as breed characteristic (Biradar et al, 2014). According to a study conducted with Pandharpuri water buffalo breed, PRL exon 1 was digested by HaeIII RE and no polymorphism was found. It was determined that Pandharpuri water buffalo breed was monomorphic for AA genotype (Madnalwar et al, 2010)

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Conclusion