Investigation of Photodynamic Therapy on Breast Cancer Cell Lines Using LaF3:Tb Nanoparticles Conjugated with Meso-tetra(4-carboxyphenyl) Porphine

Abstract

Photodynamic therapy (PDT) is a clinically appropriate therapeutic procedure with a minimum invasion that can apply a selective cytotoxic effect toward intended cells. Generally, PDT involves the administration of light and a photosensitive drug (photosensitizer). In the presence of oxygen molecules, the excited photosensitizer can produce reactive oxygen species (ROS) to kill the cancerous cells. In PDT, drug or light can administrate in high doses at short time (acute PDT) or in low doses in a period of time (metronomic PDT). In addition, to solve the problem of light access to the tissues, new modality that named Self Light PDT (SLPDT), was introduced recently. According to the studies, LaF3:Tb conjugated with meso-tetra(4-carboxyphenyl) porphine (MTCP) nanosystem can function as an efficient nanosystem in SLPDT due to the ability of energy transfer between the light-exited NPs and MTCP to emissions which can generate ROS. In this research besides synthesizing and characterizing LaF3:Tb-MTCP nanosystems, we also studied energy transfer feasibility under UV radiation between NP and PS. Afterward, we tried to design several in vitro test using breast cancer cell lines, T47D and mcf-7, to understanding LaF3:Tb conjugated MTCP potentials for SLPDT or mPDT.