Investigation of P Fimbriae Presence in Escherichia coli Strains Isolated from Urine Samples in Human, and Their Antibacterial Resistance

Abstract

Background: In Turkey, Escherichia coli causing urinary tract infections (UTI) do not have current data on antibacterial resistance, extended spectrum β-lactamase (ESBL) enzymes types and virulence factors. Objectives: In this study, the isolation of E. coli from patients with UTI, determination of antimicrobial resistance of isolates, and investigation of the presence of ESBL enzymes and pyelonephritis-associated P fimbriae types, were aimed. Methods: Isolation E. coli from urine samples and identification were performed with conventional methods. Antibiotic resistance profiles were performed with the disc diffusion test (DDT). The presence of ESBL enzyme was investigated by the double disk diffusion test (DDDT). The presence of ESBL genes and P fimbriae genes were also investigated with PCR. The Chi squared test was used to compare the relationship between antibiotic susceptibility and resistance status in ESBL producing or non-producing and P fimbriae carrying and non-carrying isolates. Results: It was determined that 50.3% and 41.9% of isolates were resistant to ciprofloxacin, trimethoprim sulfamethoxazole. It was demonstrated that 49.7% of isolates were phenotypically ESBL positive, while 59.4% were genetically positive. The percentages of genes detected with PCR were 32.3%, 31.6%, 21.1%, 2.3% for blaTEM, blaCTX, blaOXA, blaSHV, respectively. A total of 51 pap genes were detected in 27.0% (36/133) of the total isolates. Conclusions: Extended spectrum β-lactamase producing E. coli infections associated with UTI increased in last years in Turkey. Further studies should be designed to investigate plasmids of ESBL positive E. coli stains isolated from UTI and whether plasmids are responsible for ESBL production.