Investigation of non-volatile substances in the Haihong fruit wine and their lipid-lowering effect

Abstract

Sixteen kinds of non-volatile fat-soluble substances were isolated from the Haihong fruit wine by organic solvent extraction and chromatographic techniques. The structures of 13 compounds were identified by means of mass spectrometry and nuclear magnetic resonance. The relationship between the pharmacological effects of Haihong fruit wine and its active substances such as hyperoside, rutin, catechin, epicatechin, and β-sitosterol was investigated. Results showed that the contents of hyperin, rutin, catechin, epicatechin, naringin dihydrochalcone, β-sterol and other polyphenol flavonoids in the Haihong fruit wine could significantly improve the lipid metabolism of hyperlipidemic mice, increase high-density lipoprotein, reduce blood viscosity, and significantly prevent platelet aggregation and fat accumulation in the liver of mice. The strong reduction and chelation of phenolic hydroxyl groups in hyperin, catechin and rutin are the essential reasons for the pharmacological effects of the fruit wine. These substances can exert their pharmacological effects by eliminating excess free radicals of ·DPPH, ·OH, and ·O2− and by complexing metal ion catalysts which are conducive to the formation of free radicals.