Abstract
Aim. To perform a study of three-dimensional micro- and nanostructure of porous biocompatible scaffolds and quantitative analysis of nanoscale porosity parameters. Materials and methods . Three-dimensional porous scaffolds made from spidroin rS1/9 (recombinant analog of spider dragline protein) were produced by salt leaching technique. Dimensions of macropores in produced three-imensional scaffolds were in range from 200 to 400 microns. The study of three-dimensional structure of scaffolds was carried out by scanning probe nanotomography technique with the use of experimental setup combining ultramicrotome and scanning probe microscope. Results. Three-dimensional nanotomographical reconstruction of scaffold macropore wall structure is obtained. The formation of three-dimensional network of interconnected pores and channels with characteristic dimensions in range from 20 to 700 nm in the volume of macropore walls of studied scaffolds is observed. Mean pore diameter is 150 nm. Volume porosity of macropore walls is 22% while volume fraction of pores interconnected in large pore clusters is about 20% of all pore volume. Conclusion. Obtained as a result of the study quantitative characteristics of porous micro- and nanostructure of scaffolds show signifi cant degree of nanoscale porosity and percolation of macropore walls what correlates with reported high effi ciency of tissue regeneration on such scaffolds implanted in vivo. Use of scanning probe nanotomography technique for analysis of characteristics and topology of micro- and nanopore systems enables to improve effi ciency of development of novel biocompatible and biodegradable materials with predicted morphological, physical, chemical and biological characteristics.
Highlights
INVESTIGATION OF NANO- AND MICROSTRUCTURE OF BIOMATERIALS FOR REGENERATIVE MEDICINE BY METHOD OF SCANNING PROBE NANOTOMOGRAPHY
Volume porosity of macropore walls is 22% while volume fraction of pores interconnected in large pore clusters is about 20% of all pore volume
Obtained as a result of the study quantitative characteristics of porous micro- and nanostructure of scaffolds show significant degree of nanoscale porosity and percolation of macropore walls what correlates with reported high efficiency of tissue
Summary
Obtained as a result of the study quantitative characteristics of porous micro- and nanostructure of scaffolds show significant degree of nanoscale porosity and percolation of macropore walls what correlates with reported high efficiency of tissue. 4. Экспериментальная установка для низкотемпературной сканирующей зондовой нанотомографии SNOTRA, комбинирующая криоультрамикротом и сканирующий зондовый микроскоп, расположенный непосредственно в криокамере Leica EM FC6 полнять измерения поверхности блока образца непосредственно после среза при низкой температуре (до –190 °С) [13]. Как размер, степень пористости, взаимосвязанность пор в объеме материала и отношение площади поверхности пор к занимаемому объему на микро- и наноуровне имеют значительное влияние на эффективность использования носителей из биодеградируемых материалов для задач регенеративной медицины, в частности на однородность распределения клеток в матриксе и эффективность регенерации нативной ткани in vivo. Стрелками указаны области выхода обеих сенсилл [13]
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