Investigation of Mutagenic Effects Of Some Synthetic Direct Dyes In Textile Industry By Using Umu - Test

Abstract

OZET : Kontrolsuz desarjla atilan boyarmaddeler ulastiklari ortamdan besin zinciri yoluyla insana kadar ulasmakta ve insan sagligini tehdit etmektedir. Tekstil endustrisinde kullanilan sentetik boyarmaddelerin %10-20 miktari boyama islemi sonrasi atik sularla kontrolsuz olarak desarj edilmektedir. Bu calismada, sentetik direkt boyarmaddelerin genotoksik ozelligi, kisa zamanli bakteriyel test sistemi olan umu -test ( Salmonella thyphimurium TA1535/pSK1002) ile arastirildi. Boyarmaddelerin 400 µg/ml, 120 µg/ml, 40 µg/ml ve 4 µg/ml konsantrasyonlarda cozeltileri hazirlanip, S9 fraksiyonu kullanilarak boyarmaddelerin karaciger enzimleri varliginda organizmadaki biyotransformasyon etkilerine bakilmistir. Calismada Direct Yellow 86, Direct Orange 39, Direct Blue 200, Direct Yellow 142 ve Direct Red 243 sentetik boyarmaddeleri arastirildi. Arastirma sonucunda, Direct Blue 200, S9 farksiyonu varliginda 400 µg/ml ve 120 µg/ml konsantrasyonda, Direct Blue 200'un S9 fraksiyonu yoklugunda 400 µg/ml konsantrasyonda mutajenik etkiye sahip olduklari belirlendi. Anahtar Kelimeler: Genotoksik, Mutajenik, Direkt boyarmadde, Salmonella thyphimurium , Umu-Test Investigation of Mutagenic Effects of Some Synthetic Direct Dyes in Textile Industry by Using Umu-Test ABSTRACT: Dyes which are discharged without being controlled reach to the human beings by food chain and threaten their health. 10-20 percent of synthetic dyes are discharged without being controlled to the waste waters after the dyeing process. In this study, the genotoxicity of synthetic direct ve dyes are searched by umu test ( Salmonella thyphimurium TA1535/pSK1002) which is short term bacterial test system. Concentrations of 400 µg/ml, 120 µg/ml, 40 µg/ml and 4 µg/ml of dye solutions are prepared. By using S9 fraction, the biotransformation effects of the dyes in an organism at the presence of liver enzymes are researched. In this study, Direct Yellow 86, Direct Orange 39, Direct Blue 200, Direct Yellow 142 ve Direct Red 243 synthetic dyes are studied. At the end of the study, Direct Blue 200 have mutagenic effects at the presence of S9 fraction at 400 µg/ml and 120 µg/ml concentration also Direct Blue 200 has mutagenic effect at the absence of S9 fraction at 400 µg/ml concentration. Keywords: G enotoxicity, Mutagenic, Direct dyes , Salmonella thyphimurium, Umu-Test