Investigation of molecular complexes of blood serum by laser correlation spectroscopy and atomic-force microscopy

Abstract

The progression of pathological processes in the human organism is accompanied by significant changes in a number of molecular parameters of biological fluids. An intensive search for methods appropriate for diagnosing homeostatic disturbances inherent in various pathologies in the preclinical stage has been performed all over the world. Macromolecular fractions of blood serum and blood plasma with typical sizes ranging from 1 to 1000 nm are of particular research interest from the viewpoint of the development of the methods intended for the early detection of oncological and cardiovascular diseases. Considerable possibilities for studying these objects are provided by a combined approach with the use of laser correlation spectroscopy and atomic-force microscopy. The results obtained within the proposed approach have been reported in the present paper. They have demonstrated that this approach holds much promise for composition analysis of blood serum and for further development of the methods employed in the early detection of serious diseases as a whole.