Abstract
The electrochemical behavior of microencapsulated laccase with a non-conductive polymer (poly(ethyleneimine), PEI) adsorbed on a glassy carbon electrode (GC) had been investigated. A Km value of 76 μM and a sensitivity of 16.6 {plus minus} 0.1 nA/μM with a response time of 7.5 s had been determined for a laccase microencapsulated with PEI that was deposited on a bare GC electrode in a McIlvaine buffer at pH = 5.75 with p-phenylenediamine (PPD). The bioelectrode has found to have a permeability, Pm, of 4.9 E-4 cm s^-1 for PPD and too strong interactions with 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonate) acid to observe any diffusion processes. The electrochemical and enzymatic oxidation of PPD was studied under various conditions to evaluate its reversibility and stability as a suitable redox mediator. RDE experiments showed a passivation for a glassy carbon electrode modified with empty poly(ethyleneimine) microcapsules under static conditions. Under hydrodynamic conditions however, the passivation was slowed down.
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