Abstract

In this study the nanobiocomposite of Microbial cellulose/Cotton/Silver is introduced as a modern wound dressing. Microbial cellulose was synthesized in situ on cotton gauze. The static medium culture (Hestrin & Scharm) and Acetobacter Xylinum used for microbial cellulose synthesis and 6 days formed layers used for experiments. Half of 6 days samples were reverse after 3 days in order to form double coated gauze. One or two coated sides specimens were deactivated purified and dipped in a 500ppm nanosilver concentration. Specimens were analyzed by X-ray diffraction method, Fourier transform infra-red spectroscopy, Scanning electron microscopy and transmission electron microscopy. Their water and moisture absorption determined and their antibacterial efficiency evaluated by AATCC 100 antibacterial test method. Results showed about 30% increase in water absorption with less than 8% moisture regain. Microscopic images showed a proper distribution of nanosilver without agglomerations at surface and inside nanobiocomposite which caused improved antimicrobial efficiency. The obtained results indicated that nanocomposite (double coated gauze) has high potential for applying as a modern wound dressing.

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