Investigation of Membrane-Located Receptors

Abstract

#A some Strategies for Receptors: Binding, Size and Isolation.- #A-1 Where do we stand on receptors today?.- #A-2 Strategy in receptor binding studies.- #A-3 Principles of receptor binding assays: the GABA receptor.- #A-4 Specificity criteria for receptor sites in binding studies.- #A-5 Target size analysis of cell surface receptors and adenylate cyclase.- #A-6 Determination of molecular weights of receptors, in solution and in situ.- #A-7 Development and purification of calmodulin andibodies: use in elucidating multiple functions of the protein in eukaryotic cells.- #A-8 Approaches to isolating minor membrane components using monoclonal antibodies, as in purifying 5?-nucleotidase.- #A-9 Receptor purification: a brief overview.- #A-10 Isolation of purified membranes and membranous cell components for receptor studies.- #NC(A) NOTES and COMMENTS related to the foregoing topics.- #NC(A)-1 Molecular genetics applied to receptor analysis.- #B Membranes and Associated Molecules and Processes, Including Hormonal Phenomena.- #B-1 Isolation and characterization of spiny- (clathrin)-coated membranes and vesicles from rodent Liver and, role in receptor-mediated processes.- #B-2 Hormone receptor -adenylate cyclase interaction: superpotency, superaffinity and desensitization phenomena.- #B-3 Analyzing the steps in coupling the opiate receptor to adenylate cyclase in neuroblastoma x glioma hybrid cells NG108-15.- #B-4 Architecture of plasma membrane hormone receptor-effector systems.- #B-5 Epithelial sodium channels: ligand binding techniques in intact tissues and homogenates.- #B-6 Role of internal membrane-located receptors in intracellular trafficking.- #B-7 Subcellular strategies for tracing receptor-mediated ligand uptake pathways in liver.- #B-8 Receptor-mediated transfer of proteins from blood to bile.- #B-9 Involvement of microtubules in insulin-receptor turnover in cardiac myocytes.- #NC(B) NOTES and COMMENTS related to the foregoing topics.- #NC(B)-1 Thyroid membrane NAD-glycohydrolase and ADP-ribosyl-transferase, possibly relevant to TSH action.- #NC(B)-2 Isolated adenylate cyclase and ?-adrenergic receptor.- #NC(B)-3 Insulin internalization as studied by subcellular fractionation and electron-microscopic radioautography.- #NC(B)-4 Synaptosomes and synaptic membranes: isolation and morphology.- #C Homing of Circulating Peptides, Proteins and Drug Carriers.- #C-1 Characterization of cell-surface receptors for calcitonin in hormone-responsive cancer cells.- #C-2 Binding sites for pituitary growth hormone and prolactin in the liver and mammary gland of the pregnant rabbit.- #C-3 Affinity labelling of the thyrotropin receptor.- #C-4 Affinity labelling techniques for low-abundance membrane components: peptide hormone receptors and the D-glucose transporter.- #C-5 A glucose-sensitive glycoprotein recognition system on rat hepatic endothelial cells.- #C-6 Plasma lipoproteins and the regulation of cellular function.- #C-7 Liposomes targeted to cellular receptors.- #C-8 Targeted drug carriers: biological activity of the N-acetylcysteine - liposome system.- #NC(C) NOTES and COMMENTS related to the foregoing topics.- #NC(C)-1 Hepatic growth hormone/prolactin receptors in male rats: intracellular localization, induction and internalization.- #NC(C)-2 The LDL receptor: functional studies and partial characterization.- #NC(C)-3 The receptor for immunoglobulin E on mast cells and basophils.- #NC(C)-4 Preparation of immunoliposomes.- #NC(C)-5 Targeting toxins by conjugation to antibodies: the effect of the linkage group on biological activity.- #NC(C)-6 Antibody-targeted liposomes: specific cell binding and cytotoxicity.- #NC(C)-7 Liposomes as vehicles for inhibitors of intracytoplasmic protein synthesis.- #D Cholinergic and Catecholamine Receptors.- #D-1 Examination of ligand occupation and the permeability response of the nicotinic acetylcholine receptor on intact cells.- #D-2 Group-specific reagents as probes for measuring agonist-mediated conformational changes of ?-adrenergic and muscarinic acetylcholine receptors.- #D-3 Physico-chemical studies of ?-adrenergic receptors on intact cells.- #D-4 In vitro regulation of ?-adrenergic receptors.- #D-5 Characterization and isolation of brain dopamine (D2) receptors.- #NC(D) NOTES and COMMENTS related to the foregoing topics.- #NC(D)-1 Affinity labelling of a cholinergic nicotinic receptor in cultured nerve cells.- #NC(D)-2 Solubilization and characterization of muscarinic acetylcholine receptors.- #NC(D)-3 Antibodies to a covalent antagonist used to isolate the muscarinic cholinergic receptor from rat brain.- #NC(D)-4 Myasthenia gravis - some experimental approaches.- #NC(D)-5 Guanine nucleotide regulation of agonist binding to hepatic ?1-adrenergic receptors.- #NC(D)-6 Biochemical and immunological methods to purify the ?-adrenergic receptors.- #E Various Neuroreceptors.- #E-1 Solubilization and characterization of active opiate binding sites.- #E-2 Selective tolerance development facilitates a differentiation of multiple opiate receptors.- #E-3 Subcellular fractionation of insect muscle.- #E-4 The receptor for ?-latrotoxin, studied in pre-synaptic membranes and membrane extracts.- #E-5 Allosteric interactions in receptor binding studies.- #NC(E) NOTES and COMMENTS related to the foregoing topics.- #NC(E)-1 A slowly dissociating form of bovine hippocampal synaptic membrane opiate receptors.- #NC(E)-2 Binding of reversible and irreversible ligands to brain opiate receptors.- #NC(E)-3 Affinity probes for opioid receptors in synaptosomes.- #NC(E)-4 Biochemical pharmacology of substance P receptors.- #NC(E)-5 Substance P-stimulated phosphatidylinositol breakdown in guinea pig ileum.- #NC(E)-6 Soluble GABA/benzodiazepine/barbiturate receptor interactions in mammalian brain.- #NC(E)-7 Characterization and purification of the glycine receptor of rat spinal cord.- #NC(E)-8 Observations on receptor sites for dihydropyridines.- #NC(E)-9 Specific high-affinity membrane receptors for biologically active phorbol and ingenol esters.- #NC(E)-10 Molecular graphics and computational chemistry techniques for drug design.- #NC(E)-RT Methodology for neuroreceptors Round-table discussions.- Computer-aided conformational approaches to modelling.- An Editor's Guide to receptor studies with cell-free systems.- Retrieval Key (topic index, complementing the General Index).- General Index.- Corrections to Vols. 11 & 12.