Abstract
In this study, waste peach (WP) liquid culture conditions for the maintenance of high triacylglycerol (TG)-accumulation ability in Lipomyces wild-type strain, obtained from WP plate medium were investigated. As the concentration of WP juice was high, the medium viscosity became high, and TG accumulation ability was suppressed. In a 5-L jar fermenter, the negative influence of viscosity on TG-accumulation ability was significantly improved by an agitation speed of 150 rpm (0.4 vvm). Where a bench scale pilot plant (90-L jar fermenter) was operated at 40 rpm, TG-accumulation ability reached 6.8 mg/108cells. This ability was 85% of that obtained with WP plate medium.
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