Investigation of lignans and anthocyanins as the main biologically active polyphenols of Schizandra chinensis fruits

Abstract

For the first time the content and composition of dibenzocyclooctadiene lignans and anthocyanins in 10 samples of Chinese magnolia-vine fruits (Schizandra chinensis) collected in the Moscow Region, Khabarovsk and Primorsky Krai were studied. The determination of the profile of lignans was carried out according to the our original HPLC technique with diode-array spectrophotometric and mass spectrometric detection. The validation of the methodology for linearity, accuracy and precision was carried out. 11 characteristic for Schizandra chinensis lignans were found in all samples. In dried fruits the main lignan was schisandrine, in fresh-frozen fruits - angeloylgomizin O. The total content of lignans in fresh-frozen fruits was 5.50 mg/g, in dried fruits - from 12.50 to 18.95 mg/g. Anthocyanins of Chinese magnolia-vine were mainly represented by cyanidin glycosides, among which cyanidin-3-xylosylrutinoside was predominated. The total content of anthocyanins determined by pH-differential spectrophotometry was from 0.21 mg/g in fresh-frozen fruits to 0.35-0.72 mg/g in dried fruits. The results obtained can be used to identify and standardize the fruits of Chinese magnolia- vine and the products of their processing.