Abstract
Polymerase chain reaction–sequence-specific oligonucleotide probes typing procedures identifying alleles of the killer immunoglobulin–like gene (KIR2DL4) have been established. The methods, designed around the specific amplification of the D0 and D2 domains of this gene, produce discrimination of KIR2DL4 alleles. The methods have been applied to a healthy Northern Irish control group, establishing frequencies for this Caucasian population. Additionally, the KIR2DL4 allele status of cell line DNA and CEPH families, from the 13 th International Histocompatibility Workshop and local families, have also been investigated.
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