Investigation of in vivo protective effect of orally administered vitamin E and selenium against gentamicininduced renal and hepatic toxicity

Amin A Al-Doaiss,Yazun B Jarrar

doi:10.4314/tjpr.v18i7.10

Amin A Al-Doaiss, Yazun B Jarrar

Open Access

https://doi.org/10.4314/tjpr.v18i7.10

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Abstract

Purpose: To investigate the protective effect of vitamin E (Vit E) and selenium (Se) combination against gentamycin (GM)-induced renal and hepatic toxicity in rats. Methods: Forty-eight male Wistar albino rats were administrated GM at a dose of 80 mg/kg/day, with or without Se (1.5 mg/kg/day), and/or Vit E (250 mg/kg/day) for a period of 4 weeks. Serum samples from each rat were subjected to biochemical analysis for kidney and liver functions, while kidney and liver biopsies were also investigated by histological examination. Results: GM significantly increased serum creatinine, urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and free radicals (p < 0.05). Moreover, GM induced significant histological and ultrastructural alterations in the renal and hepatic tissues of the rats. Exposure to a combination of Vit E and Se did not attenuate the GM-induced toxicity in renal and hepatic tissues. Conclusion: These results suggest that Vit E and Se combination have no significant protective role against GM-induced hepatic and renal toxicity.

Highlights

Gentamicin (GM) is still considered an important aminoglycoside antibiotic which is widely used in treatment of bacterial infections caused by gram negative bacteria [1]
Co-administration of vitamin E and Se did not ameliorate the GM-induced changes in kidney and liver functions tests
After 4 weeks of treatment of the GM rats with vitamin E (Vit E) and Se, ALT and AST were more significantly elevated, relative to rats treated with GM alone (p < 0.05)

Summary

INTRODUCTION

Gentamicin (GM) is still considered an important aminoglycoside antibiotic which is widely used in treatment of bacterial infections caused by gram negative bacteria [1]. The present study was conducted to determine the in vivo influence of orallyadministered Vit E and Se on GM-induced toxicity in renal and hepatic tissues. GM-treated group with co-administration of Vit E and Se: Single daily doses of Se (1.5 mg/kg) and Vit E (250 mg/kg, dissolved in corn oil) were administrated to the rats via gavage. Vitamin E- and Se-treated: The rats in this group received daily single dose of Se (1.5 mg/kg) and single dose of Vit E (250 mg/kg) via gavage for 4 weeks. At the end of the first and fourth weeks after treatment with GM and combination of Vit E and Se, blood samples were taken from the orbital sinus of each rat, and serum was obtained for measurement of biochemical parameters of kidney function (urea and creatinine), and liver function (ALT, AST, and alkaline phosphatase, ALP). Significant changes in comparison with the control group were indicated as p < 0.05

RESULTS

DISCUSSION

CONCLUSION

Conflict of interest