Investigation of in vitro biological activities of hollow mesoporous carbon nanoparticles bearing D-NMAPPD on human lung adenocarcinoma cells

Abstract

The uniformly dispersed hollow mesoporous carbon nanoparticles (HMCNPs) were successfully synthesized by hard-template methods, and D-NMAPPD (B13) was successfully loaded onto the nanoparticle surface for the first time. Structural properties of bare and B13 loaded HMCNPs (HMCNs-B-13) were investigated by Fourier Transform Infrared Spectroscopy (FT-IR), Field Emission-Scanning Electron Microscopy (FE-SEM), Thermal Gravimetric Analysis (TG). The amount of drug released was determined via in vitro drug release studies at 37 °C in SBF through UV–Vis spectrometric and thermal analyses. TG data revealed that the proportion of loaded B-13 was 33.60%. Their ability to induce apoptosis in cultures of A549 human lung adenocarcinoma cells was investigated, and the inhibitory effect of HMCNPs-B-13 on lung cancer cell proliferation was determined in vitro. The IC50 values determined after application periods of 24 and 48 h were found to be 16.13 μg/mL and 12.96 μg/mL, respectively. The role of HMCNPs-B-13 on the morphology and ultrastructure of A549 cells was also investigated by confocal microscopy and Transmission electron microscopy (TEM) studies.