Investigation of important biochemical compounds from selected freshwater macroalgae and their role in agriculture

Zahir Shah,Syed Lal Badshah,Abdul-Hamid Emwas,Arshad Iqbal,Zamarud Shah,Mariusz Jaremko

doi:10.1186/s40538-021-00273-0

Abstract

BackgroundFreshwater macroalgae possess a number of important secondary metabolites. They are an unexplored source of medicinal compounds. In this study, three freshwater macroalgae—Chara vulgaris, Cladophora glomerata and Spirogyra crassa—were collected from the river Swat and the river Kabul in the Charsadda district of Khyber Pakhtunkhwa, Pakistan. To assess the role of freshwater macroalgae in agriculture, various experiments were performed on their extracts. Methanolic extract of the three macroalgae were first analyzed through gas chromatography–mass spectrometry (GC–MS) for the presence of important medicinal secondary metabolites. The methanol based macroalgae extracts were tested for antibacterial, insecticidal, cytotoxic and phytotoxic activities.ResultsInitially, the algae were dried, crushed and treated with methanol for the extraction of secondary metabolites. The GC–MS results contained several important long chain fatty acids and other related long-chain hydrocarbons, such as alkanes and alkenes. Several benzene derivatives were also detected during the course of the investigation. Several of these compounds have established roles in the treatment of human ailments and can be supplied to farm animals. For example, phenylephrine is a decongestant, dilates pupils, increases blood pressure and helps in relieving hemorrhoids. Hexahydropseudoionone has uses in perfumes and other cosmetics. Several essential oils were also detected in the methanolic extract of the three macroalgae that can be utilized in various industrial products. Bioassays showed that these algal extracts—especially the Spirogyra sp. extract—contain moderate to maximum bioactivity.ConclusionsMacroalgae possess important secondary metabolites with medicinal properties. These secondary metabolites can be used as biopesticides, plant growth enhancers, and remedies for various diseases in farm animals and for the control of weeds. They can be further explored for isolation and purification of useful biochemical compounds.Graphical

Highlights

Algae are a heterogeneous group of plant-like organisms that belongs to the kingdom Protista
We have investigated the chemical composition of methanolic extract from the freshwater macroalgae Chara vulgaris, Cladophora glomerata, and Spirogyra crassa, that prevail in the river Swat and Kabul
Materials and methods The freshwater macroalgae, Chara vulgaris, Cladophora glomerata and Spirogyra crassa were collected from the river Swat and river Kabul flowing through the Charsadda district, KPK state of Pakistan (Fig. 1)

Summary

Introduction

Algae are a heterogeneous group of plant-like organisms that belongs to the kingdom Protista. Growing algae convert the sun’s energy into a diverse group of metabolites called bioactive compounds. Members of the Chlorophyceae class of green algae are a source of bioactive compounds with diverse chemical structures and biological activities, with an approximately 3.98 billion dollar market in 2018 [4]. Both marine and freshwater algae are a good source of pharmacologically active metabolites [5, 6]. Freshwater macroalgae possess a number of important secondary metabolites They are an unex‐ plored source of medicinal compounds. The methanol based macroalgae extracts were tested for antibacterial, insecticidal, cytotoxic and phytotoxic activities

Methods

Results

Conclusion